Applications of Princeton Chromatography Inc.



©PCI•2002

Application Notes



	HPLC COLUMNS
	SFC COLUMNS

APPLICATIONS

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APPLICATION NOTES

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Stationary Phases



• Beta Blockers
• Antiarrthyhmic Compounds
• Diuretics
• Water Soluble Vitamins
• Analgesic Tablet - USP
• Organic Acids
• Antidepressants
• Flavonoids
• Aromatic Acids
• Steroids
• Creatine and Creatinine
• Uracil and 5-Fluorouracil
• Proteins



	• USP XXIII - Acetaminophen
	and Codeine Phosphate Tablet
	• “Combinatorial” Test Mixture
	• Vanillins
	• Bovine Serum Albumin
	• Size Exclusion of Proteins
	• Explosives
	• Taxol
	• Buckminsterfullerenes
	• SEC of Aromatic Acids
	• Size Exclusion
	Chromatography of a Polyanionic
	Polymer (Avgmw~700K)


	Note
If the item that you are seeking is not listed in the catalog, please call for information. PCI strives to add new products to meet the needs of chromatographers.

Beta Blockers

Antiarrthyhmic Compounds


large	Column:PrincetonSPHER 2-Ethylpyridine Catalog No: 150046-01577 Dimensions: 150 x 4.6 mm Eluent: 80/20 A/B A: 20mM K2HPO4 - pH 6.6 B: Acetonitrile Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV at 273 nm Injection: 10uL (1 mg/mL sample) 1. Metoprolol 2. Propanolol 3. Pindolol	large	Column: PrincetonSPHER C18 100A 5u Catalog No: 150046-03501 Dimensions: 150 x 4.6 mm Eluent: 60/40 A/B A: 20mM NH2H2PO4 - pH 3.2 B: Acetonitrile Flow Rate: 1.5 mL/min Temperature: 40 degrees C Detection: UV at 280 nm Injection: 10 uL (1 mg / mL sample) 1. Phenytoin 2. Propanolol 3. Quinidine 4. Dilatizem 5. Verapamil 6. Nicardipine
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Diuretics

Water Soluble Vitamins


large	Column: PrincetonSPHER C18 100A 5u Catalog No: 150046-03501 Dimensions: 150 x 4.6 mm Eluent: 60/40 A/B A: 20mM NH2H2PO4 - pH 3.2 B: Acetonitrile Flow Rate: 1.5 mL/min Temperature: 40 degrees C Detection: UV at 280 nm Injection: 10 uL (1 mg/mL sample) 1. Hydrochlorothiazide 2. Chlorthalidone 3. Diazoxide 4. Furosemide 5. Bendroflumethazide	large	Column: PrincetonSPHER C18 100A 5u Catalog No: 150046-03501 Dimensions: 150 x 4.6 mm Eluent: 0-5 min 100% A; 5-20 min 100% - 0% A / 0% -100% B Linear; 20-25 min 100% B A: 20mM K2HPO4 - pH 6.6 B: 80/20 K2HPO4 (pH 6.6) / ACN Flow Rate: 1.0 mL/min Temperature: 30 degrees C Detection: UV at 254 nm Injection: 10uL (1 mg/mL sample) 1. L-Ascorbic Acid 2. Nicotinic Acid 3. Pyroxidine 4. Nicotinamide 5. Folic Acid 6. Cyanocobalamin 7. Riboflavin
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Analgesic Tablet - USP

Organic Acids


large	Column: PrincetonSPHER C18 100A 5u Catalog No: 100046-03501 Dimensions: 100 x 4.6 mm Eluent: 69/28/3 A/B/C A: Water B: Methanol C: Acetic Acid Flow Rate: 2.0 mL/min Temperature: 45 degrees C Detection: UV at 254 nm Injection: 5 uL (1 mg/mL sample) 1. Acetaminophen 2. Caffeine 3. Aspirin 4. Benzoic Acid	large	Column: PrincetonSPHER OmniSEP Catalog No: 250046-01590 Dimensions: 250 x 4.6 mm Eluent: 0.1% H3PO4 Flow Rate: 1.0 mL/min Temperature: 30 degrees C Detection: UV at 210 nm Injection: 20 uL (1 mg/mL sample) 1. Tartaric Acid 2. Formic Acid 3. Malic Acid 4. Malonic Acid 5. Lactic Acid 6. Maleic Acid
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Antidepressants

Flavonoids


large	Column: PrincetonSPHER Ultima-C18-WP Catalog No: 150046-01524 Dimensions: 150 x 4.6 mm Eluent: 40/60 A/B A: 25mM KH2PO4 - pH 6.6 B: Acetonitrile Flow Rate: 2.0 mL/min Temperature: 40 degrees C Detection: UV at 230 nm Injection: 10uL (1 mg/mL sample) 1. Protriptyline 2. Desipramine 3. Nortriptyline 4. Doxepin 5. Imipramine 6. Amitriptyline 7. Trimipramine	large	Column: PrincetonSPHER FO* 60A 5u Catalog No: 250046-01542 Dimensions: 250 x 4.6 mm Eluent: 60/40 A/B A: 0.1% H3PO4 B: Acetonitrile Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 254 nm Injection: 10uL (1 mg/mL sample) 1. Quercetin 2. Naringenin *FO = Fluorooctyl
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Aromatic Acids

Steroids


large	Column: PrincetonSPHER C18 100A 5u Catalog No: 100046-03501 Dimensions: 100 x 4.6 mm Eluent: 50/50 A/B A: 0.1M H3PO4 - pH 2.0 B: Methanol Flow Rate: 1.5 mL/min Temperature: Ambient Detection: UV at 240 nm Injection: 5 uL (1 mg/mL sample) 1. p-Aminobezoic Acid 2. p-Hydroxybenzoic Acid 3. o-Aminobenzoic Acid 4. Benzyl Alcohol 5. Salicylic Acid	large	Column: PrincetonSPHER C18 100A 5u Catalog No: 250046-03501 Dimensions: 250 x 4.6 mm Eluent: 66/34 A/B A: Acetonitrile B: Water Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 200 nm Injection: 10uL (1 mg/mL sample) 1. Ethinyl Estradiol 2. Norethindrone
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Creatine and Creatinine

Uracil and 5-Fluorouracil


large	Column: PharmaBOND C18 125A 10u Catalog No: 300039-05001 Dimensions: 300 x 3.9 mm Eluent: 70/30 A/B* A: 0.1% H3PO4 B: Acetonitrile *with 1.4 mg/mL Dodecyl Sulfate Sodium Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 210 nm Injection: 5 uL (1 mg/mL sample) 1. Creatine 2. Creatinine	large	Column: PrincetonSPHER DIOL 60A 5u Catalog No: 250046-01509 Dimensions: 250 x 4.6 mm Eluent: 80/20 A/B A: Acetonitrile& B: Water Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 254 nm Injection: 10uL (1 mg/mL sample) 1. 5-Fluorouracil 2. Uracil
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Proteins

USP XXIII - Acetaminophen and Codeine Phosphate Tablet


large	Column: PrincetonPREP C18 150A 18u Catalog No: 250046-06801 Dimensions: 250 x 4.6 mm Eluent: Gradient* A: 0.1% TFA B: Acetonitrile 0.1% TFA * 0-5 min 75% A / 25% B; 5-25 min 75% A - 25% A, 25% B - 75% B linear; 25-30 min hold. Flow Rate: 1.5 mL/min Temperature: 20 degrees C Detection: UV at 210 nm Injection: 5 uL (1 mg/mL sample) 1. Ribonuclease 2. Insulin 3. Lysozyme 4. Myoglobin 5. Myoglobin Impurity	large	Column: PharmaBOND C18 125A 10u Catalog No: 300039-05001 Dimensions: 300 x 3.9 mm Eluent: 60/36/4; Methanol, water, Tetrahydrofuran w/ 0.1% H3PO4 and 4.4mg/mL Docusate Sodium Flow Rate: 1.5 mL/min Temperature: Ambient Detection: UV at 280 nm Injection: 15uL (1 mg/mL sample) Sample: 0.85 g Acetaminophen/Codeine tablet (#3) in 250 mL mobile phase 1. Acetaminophen 2. Codeine
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“Combinatorial” Test Mixture

Vanillins


large	Column: PrincetonSPHER OmniSEP Catalog No: 050046-01570 Dimensions: 50 x 4.6 mm Eluent: Gradient* A: 0.1% TFA B: Acetonitrile * 0-4 min 100% A - 0% A, 0% B - 100% B linear; 4-5 min 100% B; 5-6 min 0% A - 100% A, 100% B - 0% B. Flow Rate: 2.5 mL/min Temperature: 20 degrees C Detection: UV at 220 nm Injection: 5 uL (1 mg mL sample) 1. Sodium Nitrate 2. 1-Hydroxy-7-aza-benzotriazole 3. 4-Methoxybenzene sulfonamide 4. Methyl 3-amino- 2-thiophenecarboxylate 5. 4-Aminobenzophenone 6. Benzo[a]pyrene	large	Column: PrincetonSPHER FO* 60A 5u Catalog No: 150046-01542 Dimensions: 150 x 4.6 mm Eluent: 30/70 A/B A: Acetonitrile B: 0.1% H3PO4 Flow Rate: 1.0 mL/min Temperature: 20degrees C Detection: UV at 254 nm Injection: 5 uL (1 mg/mL sample) 1. Vanillic Acid 2. Vanillin 3. Ethyl Vanillin *FO = Fluorooctyl
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Bovine Serum Albumin

Size Exclusion of Proteins


large	Column: PrincetonSPHER SEC/Aq 300A 10u Catalog No: 300078-08072 Dimensions: 300 x 7.8 mm Eluent: 9.5 g/L Dulbecco Buffer-pH 7.3 Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 210 nm Injection: 30 uL	large	Column: PrincetonSPHER SEC/Aq 300A 10u Catalog No: 300078-08072 Dimensions: 300 x 7.8 mm Eluent: 0.015 M NaH2PO4, 0.015 M Na2HPO4, 0.15 M NaCl, pH 6.8 Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 220 nm Injection: 25 uL 1. Thyroglobulin (MW 670,000) 2. Ovalbumin (MW 44,000) 3. Cyanocobalamin (MW 1,350)
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Explosives

Taxol


large	Column: PrincetonSPHER C18 60A 5u Catalog No: 250046-01501 Dimensions: 250 x 4.6 mm Eluent: 70/30 A/B A: Acetonitrile B: Water Flow Rate: 1.5 mL/min Temperature: 30 degrees C Detection: UV at 254 nm Injection: 10 uL (1 mg/mL sample) 1. 2,4-DNT 2. 4,4’-DPA 3. 4N-DPA 4. NNO-DPA 5. 2,4-DPA 6. 2-2’-DPA 7. 2,4-DPA 8. DPA 9. 2N-DPA 10.Dibutyl Phthalate	large	Column: PrincetonSPHER PFP* 60A 5u Catalog No: 250046-01506 Dimensions: 250 x 4.6 mm Eluent: 50/50 A/B A: Acetonitrile B: Water Flow Rate: 1.0 mL/min Temperature: 30 degrees C Detection: UV at 230 nm Injection: 10 uL (1 mg/mL sample) 1. Taxol *PFP = Pentafluorophenyl
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Buckminsterfullerenes

SEC of Aromatic Acids


large	Column: PrincetonSPHER C18 60A 5u Catalog No: 250046-01501 Dimensions: 250 x 4.6 mm Eluent: 60/40 A/B A: Toluene B: Acetonitrile Flow Rate: 1.0 mL/min Temperature: 20 degrees C Detection: UV at 320 nm Injection: 5 uL (1 mg/mL sample) 1. C60 2. C70 3. C78 4. C84	large	Column: PrincetonSPHER SEC/Aq 60Å 10µ Catalog No: 300078-01072 Dimensions: 300 x 7.8 mm Eluent: 9.5 g/L Dulbecco Buffer, pH 7.0 Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV at 254 nm Injection: 10 uL (1 mg/mL sample) 1. p-Hydroxybenzoic Acid 2. p-Toluenesulfonic Acid
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Size Exclusion Chromatography of a Polyanionic Polymer (Avgmw~700K)


	large		Columns: PrincetonSPHER SEC/Aq 60Å, 120Å, 300Å, 500Å, 1000Å, 4000Å Dimensions: 300 x 7.8mm (all columns) Eluent: 9.5 g/L Dulbecco Buffer - pH 7.0 Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV @ 254 nm

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