Column: PrincetonPREP C18 150A 18u
Catalog No: 250046-06801
Dimensions: 250 x 4.6 mm
Eluent: Gradient*

A: 0.1% TFA
B: Acetonitrile 0.1% TFA * 0-5 min 75% A / 25% B; 5-25 min 75% A - 25% A, 25% B - 75% B linear; 25-30 min hold.

Flow Rate: 1.5 mL/min
Temperature: 20 degrees C
Detection: UV at 210 nm
Injection: 5 uL (1 mg/mL sample)

1. Ribonuclease
2. Insulin
3. Lysozyme
4. Myoglobin
5. Myoglobin Impurity