(Fluorooctyl) 60Å pore size 3 and 5 µm particles HPLC columns are prepared with a high quality spherical silica. The Fluorooctyl columns is an effective alternative to conventional Phenyl columns for difficult separations. The Fluorooctyl columns are also effective in the separation of natural products such as Flavanones as well as Halogen containing aromatics. PrincetonSPHER-Fluorooctyl HPLC columns are useful for differentiating Halogen bearing compounds from their non-Halogened analogs.